Enzymatic method for phenylketonuria screening using phenylalanine dehydrogenase

  • Dooley K
  • 4

    Readers

    Mendeley users who have this article in their library.
  • 22

    Citations

    Citations of this article.

Abstract

An enzymatic method using phenylalanine dehydrogenase (PheDH;EC 1.4.1.-) for the determination of phenylalanine in blood-spot specimens has been developed for the Cobas Bio centrifugal analyser. Method accuracy was established by correlation of results from blood spots with phenylalanine values obtained by quantitative aminoacid analysis on simultaneously collected plasma specimens. The phenylalanine dehydrogenase method demonstrated linearity to 2500 μmol/L and had recoveries of 89.4 ± 4.6% (mean ± SEM). The between-run precision using blood-spot specimens was 9.8% at 285 μmol/L. In the context of a routine newborn screening program, the enzymatic method was found to have a lower false-positive rate (1% vs 3%) when evaluated against the classical fluorimetric Autoanalyser method. © 1992 The Canadian Society of Clinical Chemists.

Author-supplied keywords

  • PKU
  • newborn screening
  • phenylalanine
  • phenylalanine dehydrogenase
  • phenylketonuria

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free