d-Glutamate was produced from l-glutamate by two successive cellular reactions with a glutamate racemase produced by Escherichia coli TM93 harboring a plasmid containing a glutamate racemase gene from Lactobacillus brevis ATCC 8287 and a glutamate decarboxylase produced by E. coli ATCC 11246. l-Glutamate was first racemized to dl-glutamate at pH 8.5 and l-glutamate was then decarboxylated at pH 4.2. Starting from 100 g/l of l-glutamate, 50 g/l of d-glutamate remained after 15 h reaction. © 1995.
Yagasaki, M., Azuma, M., Ishino, S., & Ozaki, A. (1995). Enzymatic production of d-glutamate from l-glutamate by a glutamate racemase. Journal of Fermentation and Bioengineering, 79(1), 70–72. https://doi.org/10.1016/0922-338X(95)92748-2