Enzymatic production of d-glutamate from l-glutamate by a glutamate racemase

  • Yagasaki M
  • Azuma M
  • Ishino S
 et al. 
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Abstract

d-Glutamate was produced from l-glutamate by two successive cellular reactions with a glutamate racemase produced by Escherichia coli TM93 harboring a plasmid containing a glutamate racemase gene from Lactobacillus brevis ATCC 8287 and a glutamate decarboxylase produced by E. coli ATCC 11246. l-Glutamate was first racemized to dl-glutamate at pH 8.5 and l-glutamate was then decarboxylated at pH 4.2. Starting from 100 g/l of l-glutamate, 50 g/l of d-glutamate remained after 15 h reaction. © 1995.

Author-supplied keywords

  • Lactobacillus
  • bioconversion
  • d-glutamate
  • decarboxylase
  • racemase

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Authors

  • Makoto Yagasaki

  • Masaki Azuma

  • Shuichi Ishino

  • Akio Ozaki

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