Enzymatic unpacking of bull sperm chromatin

56Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.
Get full text

Abstract

When isolated bull sperm chromatin is incubated with 0.1 M 2-mercaptoethanol at pH 8, an extensive proteolytic degradation of sperm histone occurs, being accompanied by a marked swelling of the chromatin masses. The degradation of sperm histone is strongly inhibited by monovalent or divalent metal ions. The protease found in isolated bull sperm chromatin possesses properties indistinguishable from those of an acrosomal protease of trypsin-type, acrosin (EC 3.4.21.10), and requires a combination of NaCl, urea and 2-mercaptoethanol for its extraction. Evidence suggests that the protease travels along chromatin strands and hydrolyzes essentially all the sperm histone molecules within the chromatin masses. © 1975.

Cite

CITATION STYLE

APA

Marushige, Y., & Marushige, K. (1975). Enzymatic unpacking of bull sperm chromatin. BBA - Enzymology, 403(1), 180–191. https://doi.org/10.1016/0005-2744(75)90020-0

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free