An enzyme from lupin seeds forming alanine derivatives of cytokinins

  • Entsch B
  • Parker C
  • Letham D
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Abstract

A new enzyme, which catalyses the conversion of the cytokinin zeatin to the alanine conjugate lupinic acid, has been partly purified from developing lupin seed (Lupinus luteus). Paired-ion, reverse phase HPLC was adapted to analyse the enzyme reaction quantitatively. The enzyme used O-acetyl-l-serine as the source of the amino acid residue, and it interacted with substrates in a ping pong bi bi mechanism. A number of adenine derivatives served as substrates, but preference was shown for compounds with high cytokinin activity. The possible role of the enzyme, tentatively called β-(9-cytokinin)alanine synthase or lupinic acid synthase, in the regulation of hormone activity is discussed. © 1983.

Author-supplied keywords

  • Leguminosae
  • Lupinus luteus
  • cytokinin metabolism
  • enzyme forming alanyl conjugates
  • purification

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Authors

  • Barrie Entsch

  • Charles W. Parker

  • David S. Letham

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