Enzyme-linked coagulation assay: A clot-based, solid-phase assay for thrombin

Citations of this article
Mendeley users who have this article in their library.
Get full text


A new, solid-phase microtiter plate assay for thrombin has been developed, using fibrinogen bound to wells of a microtiter plate and peroxidase-fibrinogen in solution as an indicator system. When small amounts of thrombin are added to the mixture, peroxidase-fibrin and plate-bound fibrin are formed, and the peroxidase-fibrin binds to the plate-bound fibrin. The amount of peroxidase-fibrin binding is proportional to the thrombin concentration and time of incubation. Using this assay, thrombin was measured at concentrations as low as 0.25 ng/ml (0.006 nm) in 150 μl of sample. In the presence of the specific inhibitors benzamidine and d-phenylalanyl-l-prolyl-l-arginine chloromethyl ketone, the thrombin activity is reduced, at relative concentrations of inhibitors consistent with their affinities and mechanisms of action. The enzyme-linked coagulation assay is generally useful as a highly sensitive and convenient alternative to conventional "clot-based" tests of coagulation. © 1985.




Doellgast, G. J., & Rothberger, H. (1985). Enzyme-linked coagulation assay: A clot-based, solid-phase assay for thrombin. Analytical Biochemistry, 147(2), 529–534. https://doi.org/10.1016/0003-2697(85)90310-0

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free