Equilibria between adsorbed and displaced segments were measured for bovine γ-globulin, catalase, and ferritin monolayers at the air/water interface. Increased electrical charge shifted equilibria in favor of the displaced segments while increased temperature favored the adsorbed segments. Calculations of segment size agreed with previous independent estimations, falling in the range 0.9-1.5 nm2. Values of the reduction in free energy when a displaced segment becomes adsorbed at zero surface pressure fell within the range 4.6-8.8 kT. © 1981.
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