Evaluation of the enantioselective possibilities of sulfated cyclodextrins for the separation of aspartyl di- and tripeptides in capillary electrophoresis

Abstract

Highly sulfated α-, β- and γ-cyclodextrins were used to investigate the simultaneous separation of isomeric α- and β-aspartyl containing di- and tripeptides as well as their enantiomers in capillary electrophoresis. Separation of the enantiomers was accomplished with the different sulfated cyclodextrin types under acidic conditions (pH 2-3). In some cases, comigration of diastereomerically related isomers or epimers resulted in incomplete separations. The results obtained with the highly sulfated cyclodextrins, having an average degree of substitution 12, were compared to those obtained with sulfated cyclodextrins with degree of substitution 4. Copyright (C) 1998 Elsevier Science B.V.