Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species

  • Kontos F
  • Petinaki E
  • Spiliopoulou I
 et al. 
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Abstract

A novel method, based on PCR Restriction Fragment Length Polymorphism Analysis (PRA) of a part of the tuf gene (370 bp), was designed for the identification of 11 staphylococcal species, including the most common staphylococcal pathogens. A total of 258 clinical isolates were validated by this assay, and the results were in concordance with those obtained by the reference method of Kloos and Schleifer. © 2003 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Staphylococcus species
  • tuf gene

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Authors

  • F. Kontos

  • E. Petinaki

  • I. Spiliopoulou

  • M. Maniati

  • A. N. Maniatis

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