We were able to partially purify a polar digitalis-like factor from rat and bovine hypothalami based on the capacity to inhibit [3H]ouabain binding to intact human erythrocytes. This factor was characterized in reference to the digitalis-like factor that we have isolated and reported on. Hypothalamic factor shared digitalis-like activities and physicochemical properties with the one derived from human urine and mammalian plasma. These findings strongly suggest that a polar digitalis-like factor identical to the circulatory factor does exist in mammalian hypothalamus. © 1989.
Goto, A., Yamada, K., Ishii, M., Yoshioka, M., Ishiguro, T., Eguchi, C., & Sugimoto, T. (1989). Existence of a polar digitalis-like factor in mammalian hypothalamus. Biochemical and Biophysical Research Communications, 161(3), 953–958. https://doi.org/10.1016/0006-291X(89)91335-1