A combination of ultrasensitive bioassay and HPLC analysis was employed to analyse the garden carrot for furocoumarins. The earlier reported negative findings were due to the relatively insensitive methods of detection. Low levels of two furocoumarins, 8-MOP and 5-MOP were detected in all parts of fresh carrot plants for the first time. Occasionally one or two other unknown photoactive compounds were also present. Diseased carrot displayed increased levels of 8-MOP and 5-MOP; psoralen was also detected. In this respect carrot is capable of a phytoalexin response similar to celery. © 1986.
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