GDPβS enhances the activation of phospholipase C caused by thrombin in human platelets: Evidence for involvement of an inhibitory GTP-binding protein

12Citations
Citations of this article
2Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Guanosine 5′-O-thiotriphosphate (GTPγS) and thrombin stimulate the activity of phospholipase C in platelets that have been permeabilized with saponin and whose inositol phospholipids have been prelabeled with [3H]inositol. Ca2+has opposite effects on the formation of [3H]inositol phosphates induced by thrombin or GTPγS. While the action of GTPγS on the formation of [3H]inositol phosphates is inhibited by Ca2+, action of thrombin is stimulated by Ca2+. Guanosine 5′-O-(2-thiodiphosphate) (GDPβS), which inhibits the function of GTP-binding proteins, also inhibits the effect of GTPγS on phospholipase C stimulation but, surprisingly, increases the effect of thrombin. Ca2+increases the inhibitory effect of GDPβS on GTPγS activation of phospholipase C, but Ca2+further enhances the stimulatory effect of GDPγS on the thrombin activation of phospholipase C. This indicates that two mechanisms are responsible for the activation of phospholipase C in platelets. A GTP-binding protein is responsible for regulation of phospholipase C induced by GTPγS, while the effect of thrombin on the stimulation of phospholipase C is independent of GTP-binding proteins. However, the effect of thrombin may be modulated by the action of an inhibitory GTP-binding protein. © 1987.

Cite

CITATION STYLE

APA

Oberdisse, E., & Lapetina, E. G. (1987). GDPβS enhances the activation of phospholipase C caused by thrombin in human platelets: Evidence for involvement of an inhibitory GTP-binding protein. Biochemical and Biophysical Research Communications, 144(3), 1188–1196. https://doi.org/10.1016/0006-291X(87)91437-9

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free