Growth of Haemophilus influenzae type b in continuous culture: Effect of dilution rate on outer-membrane protein and lipopolysaccharide expression

  • Langford P
  • Moxon E
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Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D ≥ 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib. © 1992.

Author-supplied keywords

  • Continuous culture
  • Haemophilus influenzae
  • Lipoposaccharide
  • Nutrient-limitation
  • Outer-membrane proteins

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