Growth of Haemophilus influenzae type b in continuous culture: Effect of dilution rate on outer-membrane protein and lipopolysaccharide expression

  • Langford P
  • Moxon E
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 5

    Citations

    Citations of this article.

Abstract

Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D ≥ 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib. © 1992.

Author-supplied keywords

  • Continuous culture
  • Haemophilus influenzae
  • Lipoposaccharide
  • Nutrient-limitation
  • Outer-membrane proteins

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free