Growth of Haemophilus influenzae type b in continuous culture: Effect of dilution rate on outer-membrane protein and lipopolysaccharide expression

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Abstract

Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D ≥ 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib. © 1992.

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Langford, P. R., & Moxon, E. R. (1992). Growth of Haemophilus influenzae type b in continuous culture: Effect of dilution rate on outer-membrane protein and lipopolysaccharide expression. FEMS Microbiology Letters, 93(1), 43–47. https://doi.org/10.1016/0378-1097(92)90487-9

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