Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product. © 1994.
Price, N. T., Francia, G., Hall, L., & Proud, C. G. (1994). Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B. BBA - Gene Structure and Expression, 1217(2), 207–210. https://doi.org/10.1016/0167-4781(94)90037-X