Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

  • Price N
  • Francia G
  • Hall L
 et al. 
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Abstract

Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product. © 1994.

Author-supplied keywords

  • Eukaryote
  • Guanine nucleotide exchange factor
  • Initiation factor-2B
  • Protein synthesis

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Authors

  • Nigel T. Price

  • Giulio Francia

  • Len Hall

  • Christopher G. Proud

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