Headspace gas chromatography of stimulants in urine by in-column trifluoroacetyl derivatization method

  • Tsuchihashi H
  • Nakajima K
  • Nishikawa M
 et al. 
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The analysis of stimulants in urine using a headspace gas chromatography system equipped with an in-column sample trifluoroacetylation unit was investigated. A 5-ml aliquot of urine containing stimulants was pipetted into a 20-ml autosampler vial together with 3.5 g of potassium carbonate. The vial was sealed and heated for 20 min at 80°C, then 0.8 ml of the headspace gas and N-methylbis(trifluoroacetamide) gas were injected simultaneously into the gas chromatograph equipped with a flame ionization detector and a fused-silica capillary column (DB-1, 30 m × 0.32 mm I.D., film thickness 0.25 μm), with a gas-tight syringe. Calibration graphs prepared by the absolute calibration curve method showed good linearity over the concentration range of 0.04 to 50 μg/ml for methamphetamine hydrochloride and amphetamine sulphate. The detection limits were 0.03 μg/ml for both of these compounds. © 1989.

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  • Hitoshi Tsuchihashi

  • Kunio Nakajima

  • Mayumi Nishikawa

  • Koichi Shiomi

  • Seiji Takahashi

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