Hepatic metabolism of [14C]cholesterol, vehiculated by LDL, HDL2and HDL3lipoprotein particles, has been studied in rats with a permanent biliary drainage. The lipoprotein fractions were infused individually by a jugular vien catheter and bile was collected for 180 min after the administration. At the end of this period, the animals were killed and the blood and livers were collected. The free cholesterol of the HDL2fraction was secreted into bile, mainly as bile salt, preferentially to that associated with HDL3and LDL fractions (11.7% vs. 2.3% and 0.3%, respectively). The free cholesterol of the HDL3fraction, on the other hand, was taken up by liver more quickly and in a higher proportion than that associated with other lipoprotein fractions. The label incorporation in this lipoprotein fraction was secreted earlier and not transformed into bile. The contribution of LDL-vehiculated free cholesterol to bile secretion was small and the hepatic uptake amounted to no more than 12% of the injected label. © 1989.
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