High molecular weight messenger rna in polysomes of osmotic dependent saccharomyces cerevisiae mutants

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Abstract

1. 1. A fraction of heavy polysomes has been isolated from rapidly dividing osmotic dependent yeast cells. Poly A + RNA purified from this fraction represent 30% of the total polysomal poly A + RNA and consist of high molecular weight molecules. 2. 2. Both centrifugation analysis in denaturing conditions and electron microscopic studies showed the existence of mRNA with molecular weight up to 5 × 106 dallons in polysomal fractions. 3. 3. Competition hybridization experiments suggested a considerable sequence homology between high and low molecular weight poly A + RNA purified from heavy and light polysomal fractions, respectively. 4. 4. These results suggest that in rapid growing yeast cells some of the abundant mRNA might be synthesized by a mode different from the monocistronic mRNA synthesis. © 1980.

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Venkov, P. V., Ivanov, I. G., & Petrov, P. P. (1981). High molecular weight messenger rna in polysomes of osmotic dependent saccharomyces cerevisiae mutants. International Journal of Biochemistry, 13(1), 83–90. https://doi.org/10.1016/0020-711X(81)90139-7

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