High-frequency callus formation from protoplasts of Vitis labruscana Bailey and Vitis thunbergii Sieb. et Zucc. by embedding in gellan gum

  • Mii M
  • Zou Y
  • Sugiyama T
 et al. 
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Abstract

Protoplasts were isolated from cell suspension cultures of Vitis labruscana Bailey cultivar 'Niagara' and cultured in B5 medium supplemented with 0.2 mg l-12,4-dichlorophenoxyacetic acid (2,4-D), 0.02 mg l-16-benzylaminopurine (BAP), 20 g l-1sucrose and 0.5 M glucose. The protoplasts started to divide after 4 days of culture in liquid or gellan gum-solidified media. The percentage of protoplast division at 10 days of culture in gellan gum-solidified medium was 72% which was much higher than that in liquid medium (22%). Sustained growth of colonies was only achieved in gellan gum medium by adding an equal amount of fresh liquid medium without glucose after 2 weeks of culture. Most of the colonies grew to 1 mm in diameter after 3 weeks of culture and were transferred to B5 medium supplemented with the same components as used for the initial protoplast culture, except that glucose was omitted and gellan gum was replaced by agar. High-frequency cell division and rapid colony formation were also obtained with protoplasts of Vitis thunbergii Sieb. et Zucc. using the same method except that the culture medium contained 2 mg l-1naphthaleneacetic acid (NAA) and 0.2 mg l-1BAP. Organogenesis was not observed in the protoplast-derived calli of these two species. © 1991.

Author-supplied keywords

  • Vitis labruscana
  • Vitis thunbergii
  • callus formation
  • gellan gum (Gelrite)
  • protoplast culture

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Authors

  • M. Mii

  • Y. M. Zou

  • T. Sugiyama

  • S. Yanagihara

  • M. Iizuka

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