Image analysis of photochemically derivatized and charge-coupled device-detected phenothiazines separated by thin-layer chromatography

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Abstract

Phenothiazines previously separated by thin-layer chromatography were detected on the plates by photochemical derivatization and gave different RFvalues in six solvent systems. The methanol-acetic acid (95:5, v/v) system gave the best separation and greatest chromatic and fluorescence intensities. Qualitative and quantitative information about propiomazine, acetopromazine and chlorpromazine was obtained by image analysis. Images were obtained with a charge-coupled device camera (CCD) under different conditions using both pseudocolour red-green-blue (RGB) and grey-scale tones and then filtering the light emitted when the derivative spots were excited by UV light. Calibration graphs for acetopromazine were linear from 3.94 to 22.00 mM, for chlorpromazine from 2.69 to 13.00 mM and for propiomazine from 3.31 to 15.00 mM. © 1993.

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García Sánchez, F., Navas Díaz, A., & Fernández Correa, M. R. (1993). Image analysis of photochemically derivatized and charge-coupled device-detected phenothiazines separated by thin-layer chromatography. Journal of Chromatography A, 655(1), 31–38. https://doi.org/10.1016/0021-9673(93)87006-8

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