Immobilization of flavoproteins on silicon: effect of cross-linker chain length on enzyme activity

  • Rusin K
  • Fare T
  • Stemple J
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Abstract

The effect of cross-linker chain length on the activities of choline oxidase (ChO) and glucose oxidase (GOx) immobilized on oxidized silicon wafers has been investigated for the cross-linkers N-succinimidyl 4-maleimidobutyrate (GMBS) and N-succinimidyl 6-maleimidocaproate (EMCS). Enzyme activities were determined with an indirect fluorometric assay based on the production of hydrogen peroxide. Immobilization of ChO or GOx onto oxidized silicon with either cross-linker resulted in an 86-99% loss in enzymatic activity relative to the soluble form of the flavoprotein. However, the different cross-linkers had distinctly different effects on enzyme activity: EMCS-immobilized GOx was four times more active than GMBS-immobilized GOx; EMCS-immobilized ChO had a sevenfold higher activity than GMBS-immobilized ChO. © 1992.

Author-supplied keywords

  • choline oxidase
  • covalent immobilization
  • fluorescence assay
  • glucose oxidase
  • heterobifunctional cross-linker
  • silicon dioxide

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Authors

  • Karin M. Rusin

  • Thomas L. Fare

  • Joseph Z. Stemple

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