Immunostimulatory and immunosuppressive factors in human cancer ascites fluids: Effect on the primary plaque-forming response in vitro

  • Badger A
  • Merluzzi V
  • Cooperband S
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Immunosuppressive and immunostimulatory activity of human cancer ascitic fluids has been examined using the in vitro primary plaque-forming cell response (PFR) to sheep erythrocytes (Mishell-Dutton assay). We have prepared three fractions of ascitic fluid by precipitation with ammonium sulfate. Suppressive activity occurred in the fraction which was insoluble at 50% of saturation but not those fractions which precipitated at 30 or 80% of saturation. The fractions which were precipitated at 30 and 80% were stimulatory in the assay system. Normal human serum also had suppressive activity in the fraction precipitated at 50% of saturation but not as much as was found in ascitic fluid. Serum did not yield any fractions with stimulatory activity. © 1976.

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  • A. M. Badger

  • V. J. Merluzzi

  • S. R. Cooperband

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