Immunostimulatory and immunosuppressive factors in human cancer ascites fluids: Effect on the primary plaque-forming response in vitro

  • Badger A
  • Merluzzi V
  • Cooperband S
  • 1

    Readers

    Mendeley users who have this article in their library.
  • 12

    Citations

    Citations of this article.

Abstract

Immunosuppressive and immunostimulatory activity of human cancer ascitic fluids has been examined using the in vitro primary plaque-forming cell response (PFR) to sheep erythrocytes (Mishell-Dutton assay). We have prepared three fractions of ascitic fluid by precipitation with ammonium sulfate. Suppressive activity occurred in the fraction which was insoluble at 50% of saturation but not those fractions which precipitated at 30 or 80% of saturation. The fractions which were precipitated at 30 and 80% were stimulatory in the assay system. Normal human serum also had suppressive activity in the fraction precipitated at 50% of saturation but not as much as was found in ascitic fluid. Serum did not yield any fractions with stimulatory activity. © 1976.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A. M. Badger

  • V. J. Merluzzi

  • S. R. Cooperband

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free