Improved enrichment of insect gap junctions by filtration and sonication of NaOH-extracted subcellular fractions

  • Ryerse J
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Abstract

The purification of gap junctions from insects has been hampered by low yields when starting with dissected tissues or by contamination with non-junctional structures when starting with intact insects. A method is described here involving filtration and sonication of NaOH-extracted crude membrane fractions from larvae of the lepidopteran Heliothis virescens which yields fractions containing ∼50% gap junctions and ∼7 μg total protein per g of larvae and represents an estimated 10-100 fold increase in gap junction enrichment compared with a previously described procedure (Cell Tissue Res. 274: 393-403, 1993). The remaining structures in the fractions consist of non-junctional membrane, septate junctions, lamellate bodies and amorphous material. © 1995 Pearson Professional Ltd.

Author-supplied keywords

  • Gap junction
  • Heliothis virescens
  • arthropod
  • insect

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Authors

  • J. S. Ryerse

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