Improved lectin-mediated immobilization of human red blood cells in superporous agarose beads

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Abstract

A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin-agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier. © 2002 Elsevier Science B.V. All rights reserved.

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Gottschalk, I., Gustavsson, P. E., Ersson, B., & Lundahl, P. (2003). Improved lectin-mediated immobilization of human red blood cells in superporous agarose beads. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 784(1), 203–208. https://doi.org/10.1016/S1570-0232(02)00758-4

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