Improved lectin-mediated immobilization of human red blood cells in superporous agarose beads

  • Gottschalk I
  • Gustavsson P
  • Ersson B
 et al. 
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Abstract

A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin-agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier. © 2002 Elsevier Science B.V. All rights reserved.

Author-supplied keywords

  • Cytochalasin B
  • GLUT1
  • Immobilization
  • Superporous agarose
  • Wheat germ agglutinin

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Authors

  • Ingo Gottschalk

  • Per Erik Gustavsson

  • Bo Ersson

  • Per Lundahl

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