An improved method for analysis of technetium radiolabeled antibody by size exclusion HPLC

  • Muddukrishna S
  • Jorge P
  • Machner W
 et al. 
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Abstract

A quantitative radio HPLC method for the analysis of radiolabeled99mTc compounds, in particular labeled antibodies or proteins, is described. The method is based on the quantitative re-oxidation of the reduced column absorbed99mTc species to pertechnetate using hydrogen peroxide. The pertechnetate is quantitatively eluted from the column. The method can be used as a stand alone technique for determining the true radiolabeling yield of labeled proteins/antibodies. The method has been automated for routine use and the typical analysis time is 30 min. © 1994.

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Authors

  • S. N. Muddukrishna

  • P. Jorge

  • W. Machner

  • T. R. Sykes

  • A. A. Noujaim

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