[2-13C]Acetate and [26,27-13C2]cholesterol were incubated with a hairy root culture of Ajuga reptans var. atropurpurea. The C13NMR analysis of the biosynthesized 20-hydroxyecdysone revealed that both substrates were incorporated into the phytoecdysone in an appreciable yield. In contrast, acetate, but not cholesterol, was incorporated into cyasterone and 29-norcyasterone. These results have demonstrated that this plant tissue culture is an excellent experimental system for the study of ecdysteroid biosynthesis. © 1994.
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