[2-13C]Acetate and [26,27-13C2]cholesterol were incubated with a hairy root culture of Ajuga reptans var. atropurpurea. The C13 NMR analysis of the biosynthesized 20-hydroxyecdysone revealed that both substrates were incorporated into the phytoecdysone in an appreciable yield. In contrast, acetate, but not cholesterol, was incorporated into cyasterone and 29-norcyasterone. These results have demonstrated that this plant tissue culture is an excellent experimental system for the study of ecdysteroid biosynthesis. © 1994.
Nagakari, M., Kushiro, T., Matsumoto, T., Tanaka, N., Kakinuma, K., & Fujimoto, Y. (1994). Incorporation of acetate and cholesterol into 20-hydroxyecdysone by hairy root clone of Ajuga reptans var. Atropurpurea. Phytochemistry, 36(4), 907–910. https://doi.org/10.1016/S0031-9422(00)90461-8