Increased yield of antibody-producing murine spleen cell hybridomas from fusions cultured in medium supplemented with mouse serum

  • Persson M
  • Lerner R
  • 5

    Readers

    Mendeley users who have this article in their library.
  • 1

    Citations

    Citations of this article.

Abstract

The number of monoclonal antibody-producing murine hybridomas were increased 7-10-fold in three separate fusions when supplementing the FBS-containing, or serum-free, growth factor-containing, culture medium with 5% normal mouse serum. The beneficial effect was seen in fusions of A/J and 129GIX+spleen cells, from primary and secondary responses, and when using SP2/0 or P3X63Ag8U.1 as the fusion partner. © 1990.

Author-supplied keywords

  • Monoclonal antibody
  • Mouse serum
  • Spleen cell hybridoma
  • Tissue culture

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Mats A A Persson

  • Richard A. Lerner

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free