Increased yield of antibody-producing murine spleen cell hybridomas from fusions cultured in medium supplemented with mouse serum

  • Persson M
  • Lerner R
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The number of monoclonal antibody-producing murine hybridomas were increased 7-10-fold in three separate fusions when supplementing the FBS-containing, or serum-free, growth factor-containing, culture medium with 5% normal mouse serum. The beneficial effect was seen in fusions of A/J and 129GIX+spleen cells, from primary and secondary responses, and when using SP2/0 or P3X63Ag8U.1 as the fusion partner. © 1990.

Author-supplied keywords

  • Monoclonal antibody
  • Mouse serum
  • Spleen cell hybridoma
  • Tissue culture

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  • Mats A A Persson

  • Richard A. Lerner

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