Inhibition of phosphatidylinositol phosphodiesterase activity in skeletal muscle by metal ions and drugs which block neuromuscular transmission

Abstract

The calcium-dependent phosphatidylinositol phosphodiesterase activity of skeletal muscle cytosol was determined. The enzyme was inhibited by Zn2+, Cu2+ and Pb2+ ions but Mg2+ and Mn2+ were without effect. The antimalarial drugs chloroquine and quinine and the aminoglycoside antibiotics gentamicin and neomycin all of which, like Zn2+, have been shown to block neuromuscular transmission, also inhibited the enzyme. © 1985.