Insertion sequence IS2 associated with int-constitutive mutants of bacteriophage lambda

  • Pilacinski W
  • Mosharrafa E
  • Edmundson R
 et al. 
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Abstract

We have examined mutations in bacteriophage λ called int-c, which confer elevated constitutive expression on the int gene for prophage integration. One class of mutations, which map between the b538 and bio386 endpoints, does not appear to be associated with any major chromosomal modification, whereas the second class has the IS2 insertion sequence in orientation II within the region between gene int and the b538 endpoint. All int-c mutations are within gene xis, with the possible exception of int-c548, which might be located between int and xis. The present data are most consistent with the following notion: (1) the point mutations of class one inactivatthe tIterminator signal of the pI-tIleader RNA for gene int and thus render int expression independent of the antiterminating action of the cII and cIII products, and (2) the second class of int-c mutants is constitutive for Int because the IS2 insertion, when stratigically located between int and tI, provides a new constitutive promoter for int transcription. © 1977.

Author-supplied keywords

  • EcoRI restriction fragments
  • IS2 promoter
  • electron microscopy of heteroduplexes
  • pIpromoter
  • tIterminator mutations
  • transcription
  • xis gene

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Authors

  • W. Pilacinski

  • E. Mosharrafa

  • R. Edmundson

  • J. Zissler

  • M. Fiandt

  • W. Szybalski

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