Interaction of murine monoclonal subunit-specific antibodies with phosphofructokinase-1 from Saccharomyces cerevisiae

  • Kopperschläger G
  • Kriegel T
  • Birkenmeier G
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Abstract

Murine monoclonal subunit-specific antibodies against heterooctameric phosphofructokinase-1 from Saccharomyces cerevisiae exhibiting no cross-reactivity were purified and characterized regarding complex formation with the native enzyme, immunological reactivity to the SDS-denatured subunits originating from native and proteolytically truncated phosphofructokinase, and protection against proteolytic degradation. Strong complex formation was found with one α-specific antibody. Western blotting employing different enzyme forms allowed to localize epitope sites of the α-polypeptide chain. Monoclonal antibodies protect phosphofructokinase-1 against chymotryptic degradation. © 1995.

Author-supplied keywords

  • Monoclonal antibody
  • Phosphofructokinase (yeast)
  • Proteolysis
  • Subunit-specificity

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Authors

  • Gerhard Kopperschläger

  • Thomas Kriegel

  • Gerd Birkenmeier

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