Interferon (IFN) increases cytoplasmic free calcium ([Ca2+]i) in RPMI 4788 cells, a human colon cancer cell line. Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation. A cyanine dye, dis-C3- (5) was used to determine the effects of IFN on the membrane potential in cancer cells. The depolarization was seen with IFN-gamma, but not with IFN-beta. These results taken together, suggest that the IFN-gamma and -beta induced [Ca2+]i mobilization are clearly different in their dependence on Ca2+ entry through voltage-gated Ca2+ channels.
H., I., S., F., M., M., H., A., & K., O. (1988). Interferon-gamma activates the voltage-gated calcium channel in RPMI 4788 cells. Biochemical and Biophysical Research Communications, 153(3), 1276–1281. Retrieved from http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L18767935 http://bvgcsalud.c17.net/openurl?sid=EMBASE&issn=0006291X&id=doi:&atitle=Interferon-gamma+activates+the+voltage-gated+calcium+channel+in+RPMI+4788+cells.&stitle=Biochem.+Bi