Interferon (IFN) increases cytoplasmic free calcium ([Ca2+] i) in RPMI 4788 cells, a human colon cancer cell line. Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+] i, which is the earliest event after IFN stimulation. A cyanine dye, dis-C3- (5) was used to determine the effects of IFN on the membrane potential in cancer cells. The depolarization was seen with IFN-γ, but not with IFN-β. These results taken together, suggest that the IFN-γ and-β induced [Ca2+] i mobilization are clearly different in their dependence on Ca2+ entry through voltage-gated Ca2+ channels. © 1988 Academic Press, Inc.
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