Intracellular forms of adenovirus DNA VII. Excision of viral sequences from cellular DNA in adenovirus type 2-infected KB cells

  • Baczko K
  • Neumann R
  • Doerfler W
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Abstract

The high molecular weight DNA from adenovirus type 2-infected KB cells contains viral sequences covalently linked to cell DNA. These viral sequences have been analyzed after excision from the cellular DNA using three restriction endonucleases with different specificities, Eco RI, Bam HI, and Sal I. Upon cleavage of the high molecular weight DNA from adenovirus type 2-infected KB cells with these enzymes and subsequent analysis of the generated fragments by gel electrophoresis and DNA-DNA hybridization, viral DNA sequences are found which do not coincide in size with any of the fragments of adenovirus type 2 DNA generated by the same enzyme. These viral sequences are either larger than the largest restriction endonuclease fragment of adenovirus type 2 DNA, or they are intermediate in size to virion DNA fragments. Viral sequences are also found in size classes identical to those of the corresponding virion DNA fragments. These results indicate that adenovirus DNA sequences are present in the cellular DNA from adenovirus type 2-infected cells and that these viral sequences are covalently linked to cellular DNA. The pattern in which viral sequences are found to be distributed upon cleavage with restriction endonucleases and analysis by gel electrophoresis followed by DNA-DNA hybridization to specific fragments of adenovirus type 2 DNA suggests that adenovirus type 2 DNA is integrated in fragments rather than as the intact molecule. © 1978.

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Authors

  • Knut Baczko

  • Rainer Neumann

  • Walter Doerfler

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