Iodine- and chlorine-containing oxidation agents as hydroxylating catalysts in cytochrome P-450-dependent fatty acid hydroxylation reactions in rat liver microsomes

  • Gustafsson J
  • Bergman J
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Abstract

Following the discovery that cytochrome P-450 of liver microsomes can catalyze organic hydroperoxidesupported hydroxylation of various substrates in the absence of NADPH and molecular oxygen [l-5] work in our laboratory has shown that NaC102 and NaI04 support the hydroxylation of steroid substrates in liver microsomes 1451, in partially purified cytochrome P-450 preparations obtained from liver microsomes [6] and from Bacillus megaterium [7] and in adrenocortical microsomal and mitochondrial preparations [8]. The findings that NaClOs and NaI04 may serve as oxygen donors in cytochrome P450-catalyzed hydroxylation reactions indicate that the active OXYgenated species of the cytochrome contains a single oxygen atom. In order to further examine mechanisms involved in cytochrome P450catalyzed oxygenation reactions we have in this study investigated oxidation agent-supported hydroxylations of fatty acids. As an extension of our previous work we have also tried to find more efficient hydroxylating agents with iodine as central atom and have found iodosobenzene and iodosobenzene diacetate to be highly efficient oxygen donors in w2-hydroxylation of fatty acids.

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