Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin

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Abstract

The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA. © 2004 Elsevier B.V. All rights reserved.

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Jing, W., Shuhan, S., Zhenlin, H., Fengjuan, Z., & Yiling, L. (2004). Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 811(2), 103–107. https://doi.org/10.1016/j.jchromb.2004.06.035

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