Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin

  • Jing W
  • Shuhan S
  • Zhenlin H
 et al. 
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Abstract

The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • DNA
  • Purification

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Authors

  • Wang Jing

  • Sun Shuhan

  • Hu Zhenlin

  • Zhou Fengjuan

  • Ling Yiling

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