Isolation of a cDNA encoding a novel zinc-finger protein from neuroblastoma x glioma NG108-15 cells

  • Wick M
  • Ann D
  • Lee N
 et al. 
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Abstract

A subtraction cDNA library was constructed from control hybrid NG108-15 (mouse neuroblastoma x rat glioma) cells and NG108-15 cells which had been treated for 48 h with the δ-opioid agonist d-Ala2-d-Leu5enkephalin (DADLE) to down-regulate the δ-opioid receptor on these cells. Among the clones isolated from this library was NGD16-4, a 2768-bp clone encoding a putative 64-kDa protein containing 14 tandemly repeated zinc fingers (Zf) with high homology to the Krüppel family of Zf proteins. NGD16-4 also contains a region homologous to the A element of the Krüppel Associated Box (KRAB) domain, a domain recently linked to transcriptional repression. Southern and Northern analyses indicate that NGD16-4 is derived from the mouse genome. Northern analysis also demonstrates that expression of NGD16-4 mRNA is much higher in several mouse neuroblastoma cell lines than in mouse brain or other tissues. Although the function of NGD16-4 is unclear, the expression pattern of NGD16-4 indicates a possible association with the processes of differentiation or transformation in the mouse. © 1995.

Author-supplied keywords

  • 8-opioid receptor
  • KRAB domain
  • Krüppel zinc finger
  • mouse genome
  • neuroblastoma cell
  • subtraction cDNA library

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Authors

  • Marilee J. Wick

  • David K. Ann

  • Nancy M. Lee

  • Horace H. Loh

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