Isolation, purification and characterization of infectious hypodermal and hematopoetic necrosis virus (IHHNV) from penaeid shrimp

  • Lu Y
  • Loh P
  • Brock J
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Abstract

A protocol was developed for the isolation and purification of the infectious hypodermal and hematopoietic necrosis virus (IHHNV) of penaeid shrimps. Cesium chloride-banded virus obtained from three sources of infected shrimps was found to have a buoyant density of 1.33 g/cm3. Also, electron microscopical studies employing negative stain revealed isometric particles having a size of 19 ± 1 nm. Colorimetric analysis of its nucleic acid type indicated that these particles contain only RNA. © 1989.

Author-supplied keywords

  • Infectious hypodermal hematopoietic necrosis virus
  • Isolation
  • Purification
  • Shrimp virus

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Authors

  • Yuanan Lu

  • Philip C. Loh

  • James A. Brock

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