Does the lactose carrier of Escherichia coli function as a monomer?

  • Keith Wright J
  • Weigel U
  • Lustig A
 et al. 
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Abstract

The purified lactose carrier of Escherichia coli (product of the lacY gene) is shown to be a monomer in detergent micelles of dodecyl-O-β-D-maltoside. The negative-dominant phenotype of mutant carriers (lacY-dmutants) could not be verified by measurements of the rate of galactoside transport in lacY+/Y-ddiploid strains. It is proposed that the membrane-embedded carrier functions as a monomer in galactoside-H+symport. © 1983.

Author-supplied keywords

  • Detergent micelle
  • Lactose carrier
  • Mr-value
  • Negative dominance
  • lacY-dmutant

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Authors

  • J. Keith Wright

  • Ulrich Weigel

  • Ariel Lustig

  • Hubertus Bocklage

  • Martin Mieschendahl

  • Benno Müller-Hill

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