Laser-based double beam absorption detector for high-performance liquid chromatography

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Abstract

The performance of a new double-beam absorption detector based on an all-electronic noise canceler was tested under HPLC conditions. The test compounds were chosen to be 2′-Cl-4-dimethylaminoazobenzene and 4′-Cl-4-dimethylaminoazobenzene since they have absorption peaks in both the UV and the visible region. The major properties of the detector are discussed. The detector is linear through almost 4 orders of magnitude (10-5-10-9M). The deviation between consecutive measurements is about 3% and the absorption noise level is 2 · 10-6AU, which is lower by one order of magnitude compared to commercial absorption detectors. © 1993.

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Rosenzweig, Z., & Yeung, E. S. (1993). Laser-based double beam absorption detector for high-performance liquid chromatography. Journal of Chromatography A, 645(2), 201–207. https://doi.org/10.1016/0021-9673(93)83379-7

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