A manifold and oxygen-removing apparatus for preparing anaerobic enzymes

Abstract

An apparatus is described which is useful in preparations for anaerobic enzymology and physiology experiments. It has two columns which effectively remove O2 from standard N2 and Ar. Ar with 9.4 ppm O2 was passed through the columns at 2 liters per minute and the O2 concentration was lowered to less than 0.2 ppm. Purified gas can be used in an attached manifold system for replacing the gas phase in rubber-stoppered serum vials. The apparatus has several improvements over previously described systems. It is made primarily of metal, making it more durable and able to withstand higher gas pressures than glass systems. It also contains an indicator in one of the O2-removing columns to warn of contaminating O2. © 1982.