Mass fractionation of Drosophila egg chambers

  • Jacobs-Lorena M
  • Crippa M
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Egg chambers from Drosophila ovaries may now be fractionated in large quantities suitable for biochemical work. A suspension of isolated egg chambers is prepared by subjecting ovaries to digestion with 0.067% collagenase. Egg chambers are separated in size classes (which correspond to different developmental stages) by sieving them through a column of superimposed nylon nets. The method can, in principle, be used for the separation of any particles ranging upward from 10 μm in diameter. © 1977.

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  • Marcelo Jacobs-Lorena

  • Marco Crippa

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