Metal-induced alteration of the cell membrane/cytoplasm complex studied by flow cytometry and detergent lysis

  • Zucker R
  • Elstein K
  • Easterling R
 et al. 
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Abstract

Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasm-free ("clean") nuclei. Non-ionic detergents (e.g. NP40or Triton X-100) commonly are employed to solubilize cell membranes/cytoplasm to produce "clean" nuclei. Treatment of murine erythroleukemic cells (MELC) with tri-n-butyltin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NP40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90° scatter fluorescein isothiocyanate (FITC) fluorescence and was confirmed by light microscopy. © 1988.

Author-supplied keywords

  • Cadmium
  • Cell membrane/cytoplasm solubilization
  • Flow cytometry
  • NP40
  • Tributyltin
  • Zinc

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Authors

  • Robert M. Zucker

  • Kenneth H. Elstein

  • Robert E. Easterling

  • Edward J. Massaro

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