A method for the determination of the adherence of granulocytes to microtitre plates

  • Santini G
  • Battistin S
  • De Paoli P
 et al. 
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Abstract

We report a new partially automated method for the measurement of the adherence of PMN in vitro. Adherence to a plastic surface was detected by measuring leukocyte alkaline phosphatase activity of the adherent cells, with a Titertek Multiscan system. Using three different cellular concentrations (1 × 106, 5 × 105, 2.5 × 105PMN/ml) the response curve was linear to 45 min and adhesion was maximal by 30 min. The specificity of the reaction was acceptable as was the assay reproducibility (intra-assay coefficient of variation

Author-supplied keywords

  • Adherence
  • Alkaline phosphatase
  • ELISA plate
  • Polymorphonuclear leukocyte

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Authors

  • G. F. Santini

  • S. Battistin

  • P. De Paoli

  • D. Villalta

  • M. Borean

  • G. Basaglia

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