1. The conditions under which mitochondria might catalyse a net reversal of oxidative phosphorylation are analysed. 2. Rat-liver mitochondria, incubated under such conditions, show a strongly diminished affinity for oxygen. 3. The velocity of respiration under these conditions is a hyperbolic function of the oxygen concentration. 4. The Kmfor oxygen is less than 0.1 μM at low phosphate potential, irrespective of substrate, and 1-3 μM under reversal conditions. 5. The observed kinetics can be accounted for in a simple mechanism for cytochrome oxidase action. © 1975.
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