Modulation of cholesterol 7α-hydroxylase activity by nonspecific lipid transfer protein in human liver - possibly altered regulation of its cytosolic level in patients with gallstones

  • Kawata S
  • Imai Y
  • Inada M
 et al. 
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Abstract

Nonspecific lipid transfer protein (nsLTP) partially purified from human liver stimulated human microsomal cholesterol 7α-hydroxylase activity. Addition of the nsLTP preparation to the reaction mixture enhanced the activity two-fold. Treatment of the nsLTP preparation with anti-rat nsLTP antiserum, which cross-reacts with human nsLTP, reduced the 7α-hydroxylase-stimulating ability. These observations suggested that nsLTP plays a role in regulating the 7α-hydroxylase activity in the human liver. 7α-Hydroxylase activity in eight patients with cholesterol gallstones (4.7 ± 1.6 pmol/min per mg microsomal protein) was significantly lower than that in five controls (7.9 ± 3.4) (P < 0.05). The amount of nsLTP in the cytosolic fraction (105000 × g supernatant) of human liver was determined by dot-blotting immunoquantitation with the antiserum. The cytosolic level of nsLTP in the liver of the patients (716 ± 239 cpm/3 μg protein) was higher than that in the controls (438 ± 184) although the difference between the two groups was not statistically significant. This suggested that control of the cytosolic level may be affected in patients with cholesterol gallstones. © 1991.

Author-supplied keywords

  • Bile acid biosynthesis
  • Cholesterol 7α-hydroxylase
  • Gallstone
  • Lipid transfer protein
  • nonspecific

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Authors

  • Sumio Kawata

  • Yasuharu Imai

  • Masami Inada

  • Yoshiaki Inui

  • Hiroki kakimoto

  • Kazuto Fukuda

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