Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence

  • Okazaki K
  • Tanabayashi K
  • Takeuchi K
 et al. 
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Abstract

We have cloned and determined the nucleotide sequences of the seventh gene of the Miyahara strain of mumps virus (MuV) encoding the L protein. The L gene is 6925 nucleotides in length and contains a single long open reading frame which is capable of coding for a protein of 2261 amino acids with a calculated molecular weight of 256,571 Da. The deduced amino acid sequence of the L protein of MuV showed significant homology with those of six other paramyxoviruses, human parainfluenza type 2 virus, Newcastle disease virus, Sendai virus, measles virus, human parainfluenza type 3 virus, and human respiratory syncytial virus. The predicted MuV L protein contained distinct elements thought to be essential for RNA polymerase activity. A noncoding sequence of 24 nucleotides downstream of the presumed polyadenylation site of the L gene showed significant complementarity with the leader sequence composed of 55 nucleotide at the 3' end of the genomic RNA.

Author-supplied keywords

  • Amino Acid Sequence
  • Base Sequence
  • Cloning
  • DNA
  • DNA: genetics
  • Genes
  • Molecular
  • Molecular Sequence Data
  • Mumps virus
  • Mumps virus: genetics
  • RNA Replicase
  • RNA Replicase: genetics
  • Restriction Mapping
  • Viral
  • Viral Structural Proteins
  • Viral Structural Proteins: genetics
  • Viral: genetics

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Authors

  • K Okazaki

  • K Tanabayashi

  • K Takeuchi

  • M Hishiyama

  • A Yamada

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