A multisample device for electroelution, concentration, and dialysis of proteins from fixed and stained gel slices

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Abstract

A device capable of simultaneous electrophoretic elution of up to 12 different protein samples from fixed and stained polyacrylamide gel slices is described. It uses a single dialysis membrane to trap the eluted proteins, which are recovered in volumes of less than 30 μl each. As little as 1 μg of protein per sample may be recovered with about 50% efficiency. Milligram quantities of a single protein may also be eluted from fixed and stained gels. The device may be used for both passive and electrophoretic dialysis and protein concentration. © 1982.

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Green, G. R., Poccia, D., & Herlands, L. (1982). A multisample device for electroelution, concentration, and dialysis of proteins from fixed and stained gel slices. Analytical Biochemistry, 123(1), 66–73. https://doi.org/10.1016/0003-2697(82)90623-6

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