A multisample device for electroelution, concentration, and dialysis of proteins from fixed and stained gel slices

  • Green G
  • Poccia D
  • Herlands L
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Abstract

A device capable of simultaneous electrophoretic elution of up to 12 different protein samples from fixed and stained polyacrylamide gel slices is described. It uses a single dialysis membrane to trap the eluted proteins, which are recovered in volumes of less than 30 μl each. As little as 1 μg of protein per sample may be recovered with about 50% efficiency. Milligram quantities of a single protein may also be eluted from fixed and stained gels. The device may be used for both passive and electrophoretic dialysis and protein concentration. © 1982.

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