Kinetic analysis of the NADH-dehydrogenase activity of the NAD-dependent hydrogenase (hydrogen:NAD+ oxidoreductase, EC 220.127.116.11) from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1 has been carried out. The enzyme followed a ping-pong reaction mechanism with all the substrates tested. The inhibition analysis revealed an additional coenzyme binding site in the hydrogenase molecule. The presence of the spatially independent substrate-binding sites for different electron acceptors was verified by the alternative substrate method. © 1985.
Popov, V. O., Gazaryan, I. G., Egorov, A. M., & Berezin, I. V. (1985). NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1. Kinetic studies of the NADH-dehydrogenase activity. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 827(3), 466–471. https://doi.org/10.1016/0167-4838(85)90234-1