NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1. Kinetic studies of the NADH-dehydrogenase activity

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Abstract

Kinetic analysis of the NADH-dehydrogenase activity of the NAD-dependent hydrogenase (hydrogen:NAD+ oxidoreductase, EC 1.12.1.2) from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1 has been carried out. The enzyme followed a ping-pong reaction mechanism with all the substrates tested. The inhibition analysis revealed an additional coenzyme binding site in the hydrogenase molecule. The presence of the spatially independent substrate-binding sites for different electron acceptors was verified by the alternative substrate method. © 1985.

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Popov, V. O., Gazaryan, I. G., Egorov, A. M., & Berezin, I. V. (1985). NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1. Kinetic studies of the NADH-dehydrogenase activity. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 827(3), 466–471. https://doi.org/10.1016/0167-4838(85)90234-1

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