NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1. Kinetic studies of the NADH-dehydrogenase activity

  • Popov V
  • Gazaryan I
  • Egorov A
 et al. 
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Abstract

Kinetic analysis of the NADH-dehydrogenase activity of the NAD-dependent hydrogenase (hydrogen:NAD+oxidoreductase, EC 1.12.1.2) from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1 has been carried out. The enzyme followed a ping-pong reaction mechanism with all the substrates tested. The inhibition analysis revealed an additional coenzyme binding site in the hydrogenase molecule. The presence of the spatially independent substrate-binding sites for different electron acceptors was verified by the alternative substrate method. © 1985.

Author-supplied keywords

  • (A. eutrophus)
  • Hydrogen dehydrogenase
  • NADH dehydrogenase
  • Reaction mechanism

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