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Abstract

Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method. © 1988.

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Armati, P. J., Pollard, J. D., Van Reyk, D., & Van der Lubbe, L. (1988). Neuroimmunological electron microscopy with microwave-accelerated fixation. Journal of Immunological Methods, 110(2), 267–269. https://doi.org/10.1016/0022-1759(88)90113-5

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