Neuroimmunological electron microscopy with microwave-accelerated fixation

  • Armati P
  • Pollard J
  • Van Reyk D
 et al. 
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Abstract

Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method. © 1988.

Author-supplied keywords

  • Immunoelectron microscopy
  • Microwave fixation
  • Peripheral nerve

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