Neuromedin B-like peptides in rat brain: Biochemical characterization, mechanism of release and localization in synaptosomes

  • Moody T
  • Korman L
  • O'Donohue T
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Abstract

Neuromedin B-like peptides were characterized in the rat brain. A rabbit antisera was utilized which recognized neuromedin B but not bombesin or GRP. Using gel filtration and HPLC techniques, a major and minor peak of immunoreactivity was present in rat brain extracts. In both cases the main peak of immunoreactivity coeluted with synthetic neuromedin B. The density of neuromedin B-like peptides ranged 50-fold being greatest in the olfactory bulb and hypothalamus, intermediate in the hippocampus, spinal cord, medulla/pons, pituitary, midbrain, thalamus, striatum and cortex and lowest in the cerebellum. Release studies indicated that neuromedin B-like peptides were secreted from hypothalamic, olfactory bulb and thalamic slices in a Ca++-dependent manner when KCl (75 mM) was present. Also, the neuromedin B-like peptides in the rat brain were localized to synaptosomes. These data indicate that neuromedin B-like peptides may function as regulatory peptides in the CNS distinct from bombesin/GRP. © 1986.

Author-supplied keywords

  • Characterization
  • Immunoreactivity
  • Localization
  • Neuromedin B-like peptides
  • Synaptosomes

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Authors

  • Terry W. Moody

  • Louis Y. Korman

  • Thomas L. O'Donohue

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