A new data-acquisition system for the measurement of the net water flux across epithelia

  • Dorr R
  • Kierbel A
  • Vera J
 et al. 
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Abstract

We describe a computer-based data-acquisition system designed for the measurement of net water flux across epithelia. It is based on the detection of a liquid meniscus position in a capillary tube using an electro-optical device. Then the data are digitized, transferred to the computer main memory and saved in a file. The advantages of the system are: (a) to measure net water transfer with an accuracy of 50 nl; (b) to switch from absorptive to secretory conditions in real time; (c) data are directly digitized to be analytically, graphically and statistically treated; (d) the specially developed software allows change of time intervals for data acquirement and the scale in graphic events; (e) the software also allows recall to the monitor screen of graphics from previous tests or to print them on paper; (f) a simple mechanical structure, saving maintenance and time; and (g) high reliability.

Author-supplied keywords

  • colon
  • data-acquisition
  • epithelia
  • rabbit
  • short circuit current
  • transport
  • water

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Authors

  • Ricardo A. Dorr

  • Arlinet Kierbel

  • Jorge Vera

  • Mario Parisi

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