New synthesis of a spin-labeled peptide nucleic acid and its interactions with nucleic acids

  • Cerasi A
  • Millo E
  • Ottaviani M
 et al. 
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 4

    Citations

    Citations of this article.

Abstract

A new combined solid-liquid phase synthesis method for a spin labeled peptide nucleic acid (PNA) is developed. The methodology involved initial preparation of a protected PNA on solid phase, followed by efficient solution phase coupling to a spin label containing a reactive carboxylic group. This strategy allows to maintain the integrity of the nitroxide moiety during the various steps of chemical synthesis assuring in the same time the fidelity of the hybridization assay. This compound can be used as a reporter molecule to investigate the binding of peptide nucleic acids to oligonucleotide sequences (DNA or RNA) by EPR spectroscopy. © 2003 Elsevier Ltd. All rights reserved.

Author-supplied keywords

  • EPR
  • Oligonucleotides
  • Peptide nucleic acid
  • Spin label

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Aurora Cerasi

  • Maria Francesca Ottaviani

  • Gianluca Damonte

  • Michela Cangiotti

  • Umberto Benatti

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free