Novel reconstitution and enzymatic assay of the mitochondrial tricarboxylate carrier

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Abstract

The tricarboxylate carrier from beef liver mitochondria was reconstituted into liposomes using a protocol based on the absorption of Triton X-100 to hydrophobic Amberlite XAD-2 beads. The activity of the reconstituted carrier was determined spectroscopically by measuring the citrate/isocitrate exchange with an enzymatic assay. The Km for citrate obtained with this method was 35 μm and the Ki of 1,2,3-benzenetricarboxylate was 27 μm. © 1989.

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Lüthy, R., & Azzi, A. (1989). Novel reconstitution and enzymatic assay of the mitochondrial tricarboxylate carrier. Analytical Biochemistry, 177(2), 323–326. https://doi.org/10.1016/0003-2697(89)90060-2

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